Universität Stuttgart

PhD student position in experimental systems biology

Online seit
25.06.2020 - 17:51


A PhD Student position (65% E13) is available within the newly established Systems Biology group of Prof. Dr. Stefan Legewie at the University of Stuttgart. The appointment will be initially for three years.

The University of Stuttgart is one of the leading technical universities in Germany, and provides an inspiring interdisciplinary environment at the interface of biology and engineering. The Systems Biology group combines mathematical modelling with state-of-the-art experimentation and is part of the Stuttgart Research Center Systems Biology (SRCSB), one of the largest German inter-faculty infrastructures for systems biological research (https://www.srcsb.uni-stuttgart.de/).

In our research, we closely collaborate with SRCSB groups working on cellular signaling and gene regulation to gain a better understanding of cell fate decisions in health and disease. One major focus is heterogeneity at the single-cell level with special emphasis on the TGFb/SMAD signaling pathway. We study heterogeneous decision making using a combination of high-content imaging, single-cell RNA sequencing and quantitative modeling. Based our models, we gain mechanistic insights into cellular subpopulations and cell-specific gene expression programs in heterogeneous cell populations (e.g., Strasen et al., 2018; Fritzsch et al. 2018). As a second focus, we reconstruct gene regulatory networks from experimental data based on perturbation screens and multi-layered genomics data. In particular, we focus on mechanisms of post-transcriptional gene regulation, and recently used kinetic modeling as well as machine learning approaches to uncover mechanisms of alternative splicing regulation in human cancers (e.g., Braun et al., 2018; Sutandy et al., 2018; Becker et al., 2018). Currently, we apply this expertise to better understand how cancer cells develop resistance against targeted therapies by expressing alternative splice variants, in which the modified protein lacks the target epitope. See also: https://www.imb.de/research/legewie/research

The successful applicant will closely collaborate with theoretical partners within the group to derive a systems-level understanding of biological networks. The experimental work will include genomics approaches (RNAseq, NETseq; SLAMseq), smFISH, live-cell imaging and perturbation approaches (e.g., CRISPR/Cas9 genome editing). The research will benefit from the existing SRCSB expertise and service infrastructure in imaging. Experimental results will be integrated using machine learning and kinetic modeling approaches to gain insights into cellular decision making, thereby contributing to a better understanding of cancer development and therapy resistance. The candidate will participate in teaching in systems biology and will benefit from interdisciplinary education within the SRCSB, the SimTech excellence cluster  https://www.simtech.uni-stuttgart.de/) and the Graduate Academy of the University of Stuttgart (https://www.gradus.uni-stuttgart.de/en/).

For any queries regarding the projects please contact: s.legewie@imb-mainz.de.


In our group, we put special emphasis on a collaborative environment that fosters interdisciplinary education at the interface of theory and experiment. Therefore, excellent communication skills and good team spirit are required. The candidates should be fluent in English (spoken and written). Enthusiasm for basic research questions and the ability to solve problems independently are necessary. Applicants should hold a M.Sc. in cell biology, molecular biology, biophysics or closely related areas. Experience in state-of-the-art molecular biology methods from the fields of genomics or imaging is beneficial.

The University of Stuttgart has been certified as a family-friendly university since 2012 (http://www.uni-stuttgart.de/uniundfamilie/index.en.html) and wishes to increase the share of female academic staff and, for this reason, especially welcomes applications from women. Severely challenged applicants will be given preference in the case of equal qualifications.

Application procedure (Berwerbungsunterlagen): Candidates must send a single PDF file containing a motivation letter including a description of previous research experiences, a full CV, a list of publications, scans of academic degrees (Germans: including copies of Abitur- & Diplom/Master Zeugnis) and contact details of two referees to iig@iig.uni-stuttgart.de. 


Frau Michaela Moses-Endewardt
70569 Stuttgart
+49 711 68566970

Art der Beschäftigung


Weitere Informationen

Institut / Einrichtung
Systems Biology Group
Zeitraum der Beschäftigung
nach Vereinbarung
Mittwoch, 30. September 2020 - 23:59


E13, 65%